Description: The LR3 is a long-term analog of human IGF-1, specifically designed and manufactured for mammalian cell culture to support large-scale manufacturing of recombinant biopharmaceuticals. Recombinant Human LR3 Insulin Like Growth Factor-1 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 83 amino acids and having a molecular mass of 9.1kDa.
Synonyms: R3 IGF1, R3 IGF-1, R3IGF1, R3IGF-1, LONG IGF1, LONG IGF-1, LONG R3 IGF1,
LONG R3IGF1, LONG R3 IGF-1, LONG R3IGF-1.
Source: Escherichia Coli.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Amino Acid Sequence:
Purity: Greater than 90.0% as determined by SDS-PAGE.
Lyophilized from a 0.2 μm filtered solution of 300mM HAc-NaAc, pH 6.5
Lyophilized LR3 IGF1 althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution the LR3 IGF1 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
The products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
It is recommended to reconstitute the lyophilized LR3 IGF1 in sterile 18M-cm H2O
IGF-1 (Insulin-like growth factor-1) is a major hormonal mediator of statural growth. Under regular circumstances, GH (growth hormone) binds to its receptor in the liver, and other tissues, and stimulates the synthesis/secretion of IGF-1. In target tissues, the Type 1 IGF receptor, that is
homologous to the insulin receptor, is activated by IGF-1, leading to intracellular signaling which stimulates multiple processes leading to statural growth. IGF-1 metabolic actions are partly
directed at stimulating the uptake of glucose, fatty acids, and amino acids so that metabolism supports growing tissues.
The ED50 as determined by the stimulation of protein synthesis in L6 myoblasts is less then 1mg/ml, corresponding to a specific activity of 1,000,000 units/mg.